Collaborative Project #4

We propose to develop a method to form patterned cell co-cultures using the layer-by-layer deposition of synthetic ionic polymers and without the aid of adhesive proteins/ligands such as collagen or fibronectin. The ability of surfaces to modulate cellular behavior such as adhesion, migration, proliferation and differentiation is an important facet of tissue and organ formation and in the eventual realization of functional biomaterials.

Consequently, by generating bioinert materials, we are planning to first reduce any nonspecific physiological responses and then create a truly bioactive system by reintroducing the attachment of only desired cells in a predictable fashion by using proteins, cell specific signaling molecules, or adhesion ligands, often presented in precise engineered geometries.